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mCherry antibody [HL1482]

Recombinant antibody
Anti-mCherry antibody [HL1482] used in Western Blot (WB). GTX636956
Anti-mCherry antibody [HL1482] used in Western Blot (WB). GTX636956
Anti-mCherry antibody [HL1482] used in Western Blot (WB). GTX636956
Anti-mCherry antibody [HL1482] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636956

Cat No. GTX636956

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1482

Isotype

IgG

Application

WB, ICC/IF
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length mCherry recombinant protein

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-mCherry antibody [HL1482] used in Western Blot (WB). GTX636956

GTX636956 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (GTX636956) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mCherry antibody [HL1482] used in Western Blot (WB). GTX636956

GTX636956 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 Âμg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (GTX636956) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mCherry antibody [HL1482] used in Western Blot (WB). GTX636956

GTX636956 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (GTX636956) diluted at 1:5000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.

Anti-mCherry antibody [HL1482] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636956

GTX636956 ICC/IF Image

mCherry antibody [HL1482] detects mCherry protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mCherry stained by mCherry antibody [HL1482] (GTX636956) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for mCherry antibody [HL1482] (GTX636956). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations