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mCherry antibody [HL1482]

Recombinant antibody
Anti-mCherry antibody [HL1482] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636956
Anti-mCherry antibody [HL1482] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636956
Anti-mCherry antibody [HL1482] used in Western Blot (WB). GTX636956
Anti-mCherry antibody [HL1482] used in Western Blot (WB). GTX636956

Cat. No. GTX636956

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1482

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all mCherry products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Product Note

This antibody is specific for mcherry protein, and it does not cross-react with RFP and GFP.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length mCherry recombinant protein

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-mCherry antibody [HL1482] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636956

GTX636956 IHC-P (cell pellet) Image

mCherry antibody [HL1482] detects mCherry protein at cytoplasm by immunohistochemical analysis.
Sample:Paraffin-embedded mock and mCherry tagged protein transfected 293T cell pellet.
Green: mCherry stained by mCherry antibody [HL1482] (GTX636956) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mCherry antibody [HL1482] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636956

GTX636956 ICC/IF Image

mCherry antibody [HL1482] detects mCherry protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mCherry stained by mCherry antibody [HL1482] (GTX636956) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-mCherry antibody [HL1482] used in Western Blot (WB). GTX636956

GTX636956 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (GTX636956) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mCherry antibody [HL1482] used in Western Blot (WB). GTX636956

GTX636956 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (GTX636956) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations