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mCherry antibody

Anti-mCherry antibody used in IHC (Whole mount) (IHC-Wm). GTX128508
Anti-mCherry antibody used in Western Blot (WB). GTX128508
Anti-mCherry antibody used in Immunoprecipitation (IP). GTX128508
Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128508
Anti-mCherry antibody used in Western Blot (WB). GTX128508

Cat No. GTX128508

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, PLA
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T whole cell extract , human PLK4-mCherry-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length mCherry recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Research Area

DATA IMAGES
Anti-mCherry antibody used in IHC (Whole mount) (IHC-Wm). GTX128508

GTX128508 IHC-Wm Image

mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
mCherry antibody labels mCherry-expressing cells (GTX128508) dilution: 1:100.

Anti-mCherry antibody used in Western Blot (WB). GTX128508

GTX128508 WB Image

mCherry antibody detects mCherry (full length) protein by western blot analysis.
A. 5ng recombinant mCherry
B. 10ng recombinant mCherry
C. 20ng recombinant mCherry
12% SDS-PAGE
mCherry antibody (GTX128508) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mCherry antibody used in Immunoprecipitation (IP). GTX128508

GTX128508 IP Image

mcherry antibody immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of mcherry protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of mcherry protein by 2.5 μg of mcherry antibody (GTX128508) 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody (GTX128508) diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128508

GTX128508 ICC/IF Image

mCherry antibody detects mCherry protein at mCherry fusion protien by immunofluorescent analysis.
Sample: mCherry fusion protien-transfected HeLa cells were fixed in Triton X-100 for 1 min, then 4% paraformaldehyde at RT for 30 min.
Green: mCherry protein stained by mCherry antibody (GTX128508) diluted at 1:500.
Red: mCherry is expressed in the tranfected cell . Blue: Hoechst 33342 staining.

Anti-mCherry antibody used in Western Blot (WB). GTX128508

GTX128508 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

mCherry antibody Cat No. GTX128508

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 02-Nov-2018
Score :
Application Tested : IHC-Wm
Sample : Drosophila Brain
Blocking : 10% NGS in 0.3% PBST, 25°C, 4Hr
Fixation : 4% PFA, 4°C, overnightHr
Primary Antibody : 1:500, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169