mCherry antibody

mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
mCherry antibody labels mCherry-expressing cells (GTX128508) dilution: 1:100.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

mcherry antibody immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of mcherry protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of mcherry protein by 2.5 μg of mcherry antibody (GTX128508) 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody (GTX128508) diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

mCherry antibody detects mCherry (full length) protein by western blot analysis.
A. 5ng recombinant mCherry
B. 10ng recombinant mCherry
C. 20ng recombinant mCherry
12% SDS-PAGE
mCherry antibody (GTX128508) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

mCherry antibody detects mCherry protein at mCherry fusion protein by immunofluorescent analysis.
Sample: mCherry fusion protein-transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 30 min, then Triton X-100 for 1 min.
Green: mCherry protein stained by mCherry antibody (GTX128508) diluted at 1:500.
Red: mCherry is expressed in the transfected cell . Blue: Hoechst 33342 staining.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128508) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal EMBO Rep in 2020. PMID: 31908141

The data was published in the journal EMBO Rep in 2020. PMID: 31908141

The data was published in the journal Front Cell Dev Biol in 2020.PMID: 32457908

The data was published in the journal Nat Commun in 2017.PMID: 29203870

The data was published in the journal Nat Commun in 2017.PMID: 28466843

The data was published in the journal Nat Commun in 2017.PMID: 28466843

The data was published in the journal EMBO J in 2019.PMID: 31368584

The data was published in the journal Nat Commun in 2017.PMID: 29203870