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mCherry antibody

Anti-mCherry antibody used in Immunoprecipitation (IP). GTX128509
Anti-mCherry antibody used in Western Blot (WB). GTX128509
Anti-mCherry antibody used in Western Blot (WB). GTX128509

Cat No. GTX128509

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

RFP-transfected 293T cells , mCherry-tagged protein expressing 293T cells , PLK4-mCherry-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of mCherry. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Research Area

DATA IMAGES
Anti-mCherry antibody used in Immunoprecipitation (IP). GTX128509

GTX128509 IP Image

mcherry antibody immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of mcherry protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of mcherry protein by 2.5 μg of mcherry antibody (GTX128509) 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody (GTX128509) diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-mCherry antibody used in Western Blot (WB). GTX128509

GTX128509 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mCherry antibody used in Western Blot (WB). GTX128509

GTX128509 WB Image

mCherry antibody detects mCherry (C-term) protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of RFP-transfected 293T cells
C. 30 µg whole cell lysate/extract of mCherry-tagged protein expressing 293T cells
12% SDS-PAGE
mCherry antibody (GTX128509) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169