GeneTex
United States (US)

mGluR3 antibody [C3], C-term

GTX108212_39771_20171103_WB_M_R_19011507_233.jpg
GTX108212_WB_19011507_834.jpg
GTX108212_39771_20171115_ICC_IF_R_19011507_793.jpg

Cat No. GTX108212

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

293T , A431 , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human mGluR-3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutamate Metabotropic Receptor 3,Glur3,Gprc1C,Mglur3,Mglu3,Grm3

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108212_39771_20171103_WB_M_R_19011507_233.jpg

GTX108212 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mGluR3 antibody [C3], C-term (GTX108212) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108212_WB_19011507_834.jpg

GTX108212 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431 (GTX27909)
7.5% SDS PAGE
GTX108212 diluted at 1:500

GTX108212_39771_20171115_ICC_IF_R_19011507_793.jpg

GTX108212 ICC/IF Image

mGluR3 antibody [C3], C-term detects mGluR3 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mGluR3 stained by mGluR3 antibody [C3], C-term (GTX108212) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169