Unboiled mouse tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mGluR4 antibody [HL3986] (GTX642312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mGluR4 antibody [HL3986] (GTX642312) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.