mGluR5 antibody [HL2876]

mGluR5 antibody [HL2876] detects mGluR5 protein by immunofluorescent analysis.
Sample: DIV11 rat E18 hippocampal neurons and glial cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mGluR5 stained by mGluR5 antibody [HL2876] (GTX640153) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Boiled and unboiled human tissue extract (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL2876] (GTX640153) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL2876] (GTX640153) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL2877] (GTX640153) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL2876] (GTX640153) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.