mGluR5 antibody [HL3433]

mGluR5 antibody [HL3433] detects mGluR5 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mGluR5 stained by mGluR5 antibody [HL3433] (GTX641275) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3433] (GTX641275) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3433] (GTX641275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled rat tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3433] (GTX641275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.