mGluR5 antibody [HL3509]

mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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mGluR5 antibody [HL3509] (GTX641397) detects mGluR5 protein by flow cytometry analysis.
Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer to permeabilize the cell membrane.
The cells were following stained with mGluR5 antibody [HL3509] (GTX641397) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) control at 4ºC for 1 hour.

mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse striatum.
Green: mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:250.
Red: NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Hoechst 33342 staining.

mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain (hippocampus).
mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain (striatum).
mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3509] (GTX641397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3509] (GTX641397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Boiled and unboiled human tissue extract (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3509] (GTX641397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.