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mGluR5 antibody

Anti-mGluR5 antibody used in Western Blot (WB). GTX133288
Anti-mGluR5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133288

Cat No. GTX133288

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Mouse mGluR5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glutamate receptor, metabotropic 5,6430542K11Rik,AI850523,Glu5R,Gprc1e,mGluR5,mGluR5b

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.

Database

Research Area

DATA IMAGES
Anti-mGluR5 antibody used in Western Blot (WB). GTX133288

GTX133288 WB Image

Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody (GTX133288) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mGluR5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133288

GTX133288 ICC/IF Image

mGluR5 antibody detects mGluR5 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mGluR5 stained by mGluR5 antibody (GTX133288) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169