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mLST8 / GBL antibody

Anti-mLST8 / GBL antibody used in Western Blot (WB). GTX132580
Anti-mLST8 / GBL antibody used in IHC (Paraffin sections) (IHC-P). GTX132580
Anti-mLST8 / GBL antibody used in Western Blot (WB). GTX132580
Anti-mLST8 / GBL antibody used in IHC (Paraffin sections) (IHC-P). GTX132580

Cat No. GTX132580

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human mLST8 / GBL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MTOR associated protein, LST8 homolog,GBL,GbetaL,LST8,POP3,WAT1

Cellular Localization

Cytoplasm

Background

Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

Database

Research Area

DATA IMAGES
Anti-mLST8 / GBL antibody used in Western Blot (WB). GTX132580

GTX132580 WB Image

Wild-type (WT) and mLST8 / GBL knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with mLST8 / GBL antibody (GTX132580) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-mLST8 / GBL antibody used in IHC (Paraffin sections) (IHC-P). GTX132580

GTX132580 IHC-P Image

mLST8 / GBL antibody detects mLST8 / GBL protein at cytoplasm in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
mLST8 / GBL antibody (GTX132580) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mLST8 / GBL antibody used in Western Blot (WB). GTX132580

GTX132580 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with mLST8 / GBL antibody (GTX132580) diluted at 1:1000.

Anti-mLST8 / GBL antibody used in IHC (Paraffin sections) (IHC-P). GTX132580

GTX132580 IHC-P Image

mLST8 / GBL antibody detects mLST8 / GBL protein at cytoplasm in mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
mLST8 / GBL antibody (GTX132580) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf