GeneTex
United States (US)

mTOR antibody [C1], Internal

KO/KD
GTX101558_39721_20160616_WB_shRNA_watermark_19011506_415.jpg
GTX101558_39721_20151015_WB_R_brain_19011506_156.jpg
GTX101558_39721_20150515_WB_Merged_S_19011506_397.jpg
GTX101558_39721_20151029_WB_M_brain_19011506_972.jpg

Cat No. GTX101558

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

Positive Control

HeLa , Jurkat , Raji , NCI-H929 , Rat brain , Mouse brain , 293T

Predict Reactivity

Zebrafish, Sheep(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human mTOR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mechanistic Target Of Rapamycin Kinase,Frap,Frap1,Frap2,Raft1,Rapt1,Sks,Mtor

Cellular Localization

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101558_39721_20160616_WB_shRNA_watermark_19011506_415.jpg

GTX101558 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal (GTX101558) diluted at 1:500.

GTX101558_39721_20151015_WB_R_brain_19011506_156.jpg

GTX101558 WB Image

mTOR antibody [C1], Internal detects mTOR protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal (GTX101558) diluted at 1:1000.

GTX101558_39721_20150515_WB_Merged_S_19011506_397.jpg

GTX101558 WB Image

mTOR antibody detects mTOR protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GTX101558) diluted at a dilution of 1:1000.

GTX101558_39721_20151029_WB_M_brain_19011506_972.jpg

GTX101558 WB Image

mTOR antibody [C1], Internal detects mTOR protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal (GTX101558) diluted at 1:500.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169