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mTOR antibody [C1], Internal

KO/KD
Anti-mTOR antibody [C1], Internal used in Western Blot (WB). GTX101558
Anti-mTOR antibody [C1], Internal used in Western Blot (WB). GTX101558
Anti-mTOR antibody [C1], Internal used in Western Blot (WB). GTX101558
Anti-mTOR antibody [C1], Internal used in Western Blot (WB). GTX101558

Cat. No. GTX101558

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all mTOR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

Positive Control

HeLa , Jurkat , Raji , NCI-H929 , rat brain , mouse brain , 293T

Predict Reactivity

Zebrafish, Sheep(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human mTOR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241081

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS

Cellular Localization

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-mTOR antibody [C1], Internal used in Western Blot (WB). GTX101558

GTX101558 WB Image

mTOR antibody [C1], Internal detects mTOR protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal (GTX101558) diluted at 1:1000.

Anti-mTOR antibody [C1], Internal used in Western Blot (WB). GTX101558

GTX101558 WB Image

mTOR antibody [C1], Internal detects mTOR protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal (GTX101558) diluted at 1:500.

Anti-mTOR antibody [C1], Internal used in Western Blot (WB). GTX101558

GTX101558 WB Image

mTOR antibody detects mTOR protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GTX101558) diluted at a dilution of 1:1000.

Anti-mTOR antibody [C1], Internal used in Western Blot (WB). GTX101558

GTX101558 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal (GTX101558) diluted at 1:500.

REFERENCE

Application Reference

Lee KH et al. Sci Rep 2014; 4 : 6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169