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mTOR antibody [GT649]

Anti-mTOR antibody [GT649] used in Western Blot (WB). GTX630198
Anti-mTOR antibody [GT649] used in Western Blot (WB). GTX630198
Anti-mTOR antibody [GT649] used in IHC (Paraffin sections) (IHC-P). GTX630198
Anti-mTOR antibody [GT649] used in Western Blot (WB). GTX630198

Cat No. GTX630198

Host

Mouse

Clonality

Monoclonal

Clone Name

GT649

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Zebrafish, Sheep(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888200

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS

Cellular Localization

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-mTOR antibody [GT649] used in Western Blot (WB). GTX630198

GTX630198 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT649] (GTX630198) diluted at 1:500.

Anti-mTOR antibody [GT649] used in Western Blot (WB). GTX630198

GTX630198 WB Image

mTOR antibody [GT649] detects mTOR protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
mTOR antibody [GT649] (GTX630198) dilution: 1:1000

Anti-mTOR antibody [GT649] used in IHC (Paraffin sections) (IHC-P). GTX630198

GTX630198 IHC-P Image

mTOR antibody [GT649] detects mTOR protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
mTOR antibody [GT649] (GTX630198) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mTOR antibody [GT649] used in Western Blot (WB). GTX630198

GTX630198 WB Image

mTOR antibody [GT649] detects mTOR protein by western blot analysis.
A Jurkat
BRaji whole cell lysate/extract
C NCI-H929 whole cell lysate/extract
5 % SDS-PAGE
mTOR antibody [GT649] (GTX630198) dilution: 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169