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mTOR antibody [GT778]

KO/KD
Anti-mTOR antibody [GT778] used in Western Blot (WB). GTX630200
Anti-mTOR antibody [GT778] used in Western Blot (WB). GTX630200
Anti-mTOR antibody [GT778] used in Western Blot (WB). GTX630200

Cat. No. GTX630200

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT778

Isotype

 IgG2b

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all mTOR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888202

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS

Cellular Localization

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-mTOR antibody [GT778] used in Western Blot (WB). GTX630200

GTX630200 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT778] (GTX630200) diluted at 1:500.

Anti-mTOR antibody [GT778] used in Western Blot (WB). GTX630200

GTX630200 WB Image

mTOR antibody [GT778] detects mTOR protein by western blot analysis.
A Jurkat whole cell lysate/extract
BRaji whole cell lysate/extract
C NCI-H929 whole cell lysate/extract
5 % SDS-PAGE
mTOR antibody [GT778] (GTX630200) dilution: 1:1000

Anti-mTOR antibody [GT778] used in Western Blot (WB). GTX630200

GTX630200 WB Image

mTOR antibody [GT778] detects mTOR protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
mTOR antibody [GT778] (GTX630200) dilution: 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations