mTOR antibody [HL2216]

Cat. No. GTX638220
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Cat. No.  GTX638220
Recombinant antibody KO/KD Orthogonal Carrier-Free
Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
GTX638220 WB Image
KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
GTX638220 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
GTX638220 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
GTX638220 WB Image
Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

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Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
GTX638220 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-mTOR antibody [HL2216] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638220
GTX638220 ICC/IF Image

mTOR antibody [HL2216] detects mTOR protein at Golgi apparatus and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mTOR stained by mTOR antibody [HL2216] (GTX638220) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-mTOR antibody [HL2216] used in IHC (Paraffin sections) (IHC-P). GTX638220
GTX638220 IHC-P Image

mTOR antibody [HL2216] detects mTOR protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
mTOR stained by mTOR antibody [HL2216] (GTX638220) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220
Anti-mTOR antibody [HL2216] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638220
Anti-mTOR antibody [HL2216] used in IHC (Paraffin sections) (IHC-P). GTX638220
This mTOR antibody [HL2216] (GTX638220) is a rabbit monoclonal antibody validated for detection of mTOR by WB, ICC/IF, IHC-P, and IP. Reactivity has been shown in Human, Mouse, and Rat. Knockout/knockdown validation supports antibody specificity.
  • Host
    Rabbit
  • Clonality
    Monoclonal
  • Clone Name
    HL2216
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P IP
  • Reactivity
    Human, Mouse, Rat
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