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mTOR antibody [HL2217]

Anti-mTOR antibody [HL2217] used in Western Blot (WB). GTX638221
Anti-mTOR antibody [HL2217] used in Western Blot (WB). GTX638221
Anti-mTOR antibody [HL2217] used in Western Blot (WB). GTX638221
Anti-mTOR antibody [HL2217] used in IHC (Paraffin sections) (IHC-P). GTX638221
Anti-mTOR antibody [HL2217] used in Western Blot (WB). GTX638221

Cat. No. GTX638221

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2217

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human mTOR.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS

Cellular Localization

Endoplasmic reticulum membrane , Golgi apparatus membrane , Mitochondrion outer membrane , Lysosome , Cytoplasm

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-mTOR antibody [HL2217] used in Western Blot (WB). GTX638221

GTX638221 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2217] (GTX638221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mTOR antibody [HL2217] used in Western Blot (WB). GTX638221

GTX638221 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2217] (GTX638221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mTOR antibody [HL2217] used in Western Blot (WB). GTX638221

GTX638221 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2217] (GTX638221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-mTOR antibody [HL2217] used in IHC (Paraffin sections) (IHC-P). GTX638221

GTX638221 IHC-P Image

mTOR antibody [HL2217] detects mTOR protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
mTOR stained by mTOR antibody [HL2217] (GTX638221) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mTOR antibody [HL2217] used in Western Blot (WB). GTX638221

GTX638221 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2217] (GTX638221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for mTOR antibody [HL2217] (GTX638221). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for mTOR antibody [HL2217] (GTX638221). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations