APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:100 - 1:500 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human MTOR(Accession P42345), corresponding to amino acid residues M2432-D2459._x000D_
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS
Cellular Localization
Endoplasmic reticulum membrane , Golgi apparatus membrane , Mitochondrion outer membrane , Lysosome , Cytoplasm
Background
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]
Database
Research Area
DATA IMAGES
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GTX04334 IHC-P Image
IHC-P analysis of mouse ovary tissue using GTX04334 mTOR antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX04334 IHC-P Image
IHC-P analysis of rat stomach tissue using GTX04334 mTOR antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX04334 IHC-P Image
IHC-P analysis of rat testis tissue using GTX04334 mTOR antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX04334 IHC-P Image
IHC-P analysis of rat brain tissue using GTX04334 mTOR antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX04334 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX04334 mTOR antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
|
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GTX04334 WB Image
WB analysis of mouse spleen tissue lysate using GTX04334 mTOR antibody. The lane on the left was treated with blocking peptide.
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GTX04334 IHC-P Image
IHC-P analysis of mouse heart tissue using GTX04334 mTOR antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX04334 IHC-P Image
IHC-P analysis of rat liver tissue using GTX04334 mTOR antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
|
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GTX04334 IHC-P Image
IHC-P analysis of rat ovary tissue using GTX04334 mTOR antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
|
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|
GTX04334 IHC-P Image
IHC-P analysis of rat lung tissue using GTX04334 mTOR antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX04334 WB Image
WB analysis of RAW264.7 cell lysates with serum starvation treatment using GTX04334 mTOR antibody. The lane on the left was treated with blocking peptide.
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GTX04334 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX04334 mTOR antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
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GTX04334 WB Image
WB analysis of B16F10 whole cell lysate using GTX04334 mTOR antibody. The lane on the left was treated with blocking peptide.
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GTX04334 IHC-P Image
IHC-P analysis of rat spleen tissue using GTX04334 mTOR antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
|
REFERENCE
There are currently no references for mTOR antibody (GTX04334). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for mTOR antibody (GTX04334). Be the first to share your experience with this product.
