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mTOR antibody

Anti-mTOR antibody used in IHC (Paraffin sections) (IHC-P). GTX48628
Anti-mTOR antibody used in Western Blot (WB). GTX48628
Anti-mTOR antibody used in IHC (Paraffin sections) (IHC-P). GTX48628

Cat. No. GTX48628

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
25 μg ($399)
See all mTOR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250 - 1:2000
ICC/IF 1:250 - 1:2000
IHC-P 2.5 μg/ml
ELISA 1:4000 - 1:20000
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate pH7.2, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide 2440-2457 from human mTOR.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus membrane,Mitochondrion outer membrane,Lysosome,Cytoplasm

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-mTOR antibody used in IHC (Paraffin sections) (IHC-P). GTX48628

GTX48628 IHC-P Image

IHC-P analysis of human testis tissue using GTX48628 mTOR antibody.
Dilution : 5 μg/ml
Antigen retrieval : Heat-induced antigen retrieval

Anti-mTOR antibody used in Western Blot (WB). GTX48628

GTX48628 WB Image

WB analysis of HEK293 cell lysate using GTX48628 mTOR antibody.
Loading : 30μg
Dilution : 1:650

Anti-mTOR antibody used in IHC (Paraffin sections) (IHC-P). GTX48628

GTX48628 IHC-P Image

IHC-P analysis of human prostate tissue using GTX48628 mTOR antibody.
Dilution : 5 μg/ml
Antigen retrieval : Heat-induced antigen retrieval

REFERENCE

Application Reference

Alcigir ME et al. J Arthropod Borne Dis 2022; 16 (4) : 288-300 The Evaluation of Androctonus crassicauda Antivenom against the Effects of Aegaeobuthus nigrocinctus Scorpion Venom on Autophagy, Apoptosis and Necroptosis.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399