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mTOR antibody

Anti-mTOR antibody used in Western Blot (WB). GTX50541
Anti-mTOR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50541
Anti-mTOR antibody used in IHC (Paraffin sections) (IHC-P). GTX50541

Cat. No. GTX50541

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all mTOR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 2446~2450 (T-D-S-Y-S) derived from human mTOR.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus membrane,Mitochondrion outer membrane,Lysosome,Cytoplasm

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-mTOR antibody used in Western Blot (WB). GTX50541

GTX50541 WB Image

WB analysis of extracts from HeLa, 293, and JK cells using GTX50541 mTOR antibody.

Anti-mTOR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50541

GTX50541 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF-7 cells using GTX50541 mTOR antibody.

Anti-mTOR antibody used in IHC (Paraffin sections) (IHC-P). GTX50541

GTX50541 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50541 mTOR antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399