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mTOR antibody

Anti-mTOR antibody used in IHC (Paraffin sections) (IHC-P). GTX50541
Anti-mTOR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50541
Anti-mTOR antibody used in Western Blot (WB). GTX50541

Cat No. GTX50541

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 2446~2450 (T-D-S-Y-S) derived from human mTOR.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus membrane,Mitochondrion outer membrane,Lysosome,Cytoplasm

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-mTOR antibody used in IHC (Paraffin sections) (IHC-P). GTX50541

GTX50541 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50541 mTOR antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-mTOR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50541

GTX50541 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF-7 cells using GTX50541 mTOR antibody.

Anti-mTOR antibody used in Western Blot (WB). GTX50541

GTX50541 WB Image

WB analysis of extracts from HeLa, 293, and JK cells using GTX50541 mTOR antibody.

REFERENCE

There are currently no references for mTOR antibody (GTX50541). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for mTOR antibody (GTX50541). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf