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mTOR (phospho Ser2448) antibody

Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803
Anti-mTOR (phospho Ser2448) antibody used in IHC (Paraffin sections) (IHC-P). GTX132803
Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803

Cat No. GTX132803

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

Positive Control

NIH-3T3 (starvation for 16 hr) , NIH-3T3 (starvation for 16 hr and 200 ng/ml EGF treatment for 0.5 hr) , 293T , 293T (200 ng/ml EGF treatment for 0.5 hr)

Predict Reactivity

Rat, Sheep(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human mTOR (phospho Ser2448). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

mechanistic target of rapamycin kinase,FRAP,FRAP1,FRAP2,RAFT1,RAPT1,SKS

Cellular Localization

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803

GTX132803 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

Anti-mTOR (phospho Ser2448) antibody used in IHC (Paraffin sections) (IHC-P). GTX132803

GTX132803 IHC-P Image

mTOR (phospho Ser2448) antibody detects mTOR (phospho Ser2448) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).
Sample: Paraffin-embedded human lung cancer.
mTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803

GTX132803 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:1000.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf