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mouse DHFR protein, His tag (active)

mouse DHFR protein, His tag (active). GTX66970-pro

Cat No. GTX66970-pro

Application

Functional Assay

Species

Mouse
Package
100 μg ($289)

APPLICATION

Application Note

Specific activity is > 2000 pmol/min/μg, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25ºC.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-HCl buffer (pH 8.0) with 10% glycerol, 2 mM DTT, 100 mM NaCl.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 207 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD

Expression System

E.coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dihydrofolate reductase , 8430436I03Rik , AA607882 , AI662710 , AW555094

Database

Research Area

DATA IMAGES

mouse DHFR protein, His tag (active). GTX66970-pro

GTX66970-pro Image

3 μg of GTX66970-pro mouse DHFR protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf