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mtPAP antibody [1D3]

Anti-mtPAP antibody [1D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70156
Anti-mtPAP antibody [1D3] used in IHC (Paraffin sections) (IHC-P). GTX70156
Anti-mtPAP antibody [1D3] used in Western Blot (WB). GTX70156
Anti-mtPAP antibody [1D3] used in Western Blot (WB). GTX70156

Cat. No. GTX70156

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all MTPAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , MCF-7

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human mtPAP.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_11164518

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial poly(A) polymerase , PAPD1 , SPAX4 , TENT6

Cellular Localization

Cytoplasm , Mitochondrion

Background

The protein encoded by this gene is a member of the DNA polymerase type-B-like family. This enzyme synthesizes the 3' poly(A) tail of mitochondrial transcripts and plays a role in replication-dependent histone mRNA degradation.[provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-mtPAP antibody [1D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70156

GTX70156 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using mtPAP(GTX70156) antibody at 1:100 dilution.

Anti-mtPAP antibody [1D3] used in IHC (Paraffin sections) (IHC-P). GTX70156

GTX70156 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hepatoma, using mtPAP(GTX70156) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-mtPAP antibody [1D3] used in Western Blot (WB). GTX70156

GTX70156 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with mtPAP antibody [1D3] (GTX70156) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mtPAP antibody [1D3] used in Western Blot (WB). GTX70156

GTX70156 WB Image

The data was published in the journal Hum Mol Genet in 2014. PMID: 25008111

REFERENCE

Application Reference

Van Eyck L et al. Neuropediatrics 2019; (Epub) Biallelic Mutations in MTPAP Associated with a Lethal Encephalopathy.
Application : WB
Reactivity : Human
Wilson WC et al. Hum Mol Genet 2014; A human mitochondrial poly(A) polymerase mutation reveals the complexities of post-transcriptional mitochondrial gene expression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399