Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with mtPAP antibody [1D3] (GTX70156) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Hepatoma, using mtPAP(GTX70156) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using mtPAP(GTX70156) antibody at 1:100 dilution.

The data was published in the journal Hum Mol Genet in 2014. PMID: 25008111