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mtPAP antibody [N1C3]

Anti-mtPAP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118738
Anti-mtPAP antibody [N1C3] used in Western Blot (WB). GTX118738

Cat. No. GTX118738

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MTPAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

HCT116

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human mtPAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10630220

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial poly(A) polymerase , PAPD1 , SPAX4 , TENT6

Background

The protein encoded by this gene is a member of the DNA polymerase type-B-like family. This enzyme synthesizes the 3' poly(A) tail of mitochondrial transcripts and plays a role in replication-dependent histone mRNA degradation.

Database

Research Area

DATA IMAGES

Anti-mtPAP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118738

GTX118738 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Mt PAP(GTX118738) antibody at 1:200 dilution.

Anti-mtPAP antibody [N1C3] used in Western Blot (WB). GTX118738

GTX118738 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX118738 diluted at 1:5000

REFERENCE

Application Reference

Wang DD et al. J Biol Chem 2014; Helicase SUV3, Polynucleotide Phosphorylase PNPase, and Mitochondrial Polyadenylation Polymerase mtPAP Form a Transient Complex to Modulate Mitochondrial mRNA Polyadenylated Tail Lengths in Response to Energetic Changes.
Application : IP
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169