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mtRNA polymerase antibody [N2N3]

Anti-mtRNA polymerase antibody [N2N3] used in Western Blot (WB). GTX105137
Anti-mtRNA polymerase antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX105137
Anti-mtRNA polymerase antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105137
Anti-mtRNA polymerase antibody [N2N3] used in Western Blot (WB). GTX105137

Cat. No. GTX105137

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all mtRNA polymerase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human mtRNA polymerase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037731

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase mitochondrial , APOLMT , MTRNAP , MTRPOL , h-mtRPOL

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-mtRNA polymerase antibody [N2N3] used in Western Blot (WB). GTX105137

GTX105137 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
5% SDS PAGE
GTX105137 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mtRNA polymerase antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX105137

GTX105137 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using mtRNA polymerase(GTX105137) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-mtRNA polymerase antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105137

GTX105137 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep G2, using mtRNA polymerase(GTX105137) antibody at 1:500 dilution.

Anti-mtRNA polymerase antibody [N2N3] used in Western Blot (WB). GTX105137

GTX105137 WB Image

The data was published in the 2013 in Nucleic Acids Res. PMID: 23982517

REFERENCE

Application Reference

Thakur J et al. Genes Dev 2018; 32 (1) : 20-25 Unexpected conformational variations of the human centromeric chromatin complex.
Kasinathan S et al. Mol Biol Evol 2018; (Epub) Non-B-form DNA is enriched at centromeres.
Reactivity : Human
Kasinathan S et al. bioRxiv 2017; (Epub) Non-B-form DNA structures mark centromeres.
Sobek S et al. Nucleic Acids Res 2013; 41 (21) : 9848-57 Negative regulation of mitochondrial transcription by mitochondrial topoisomerase I.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169