GeneTex
United States (US)

mtRNA polymerase antibody [N2N3]

GTX105137_WB_19011506_192.jpg
GTX105137_40408_IHC_19011506_248.jpg
GTX105137_39925_IFA_19011506_649.jpg

Cat No. GTX105137

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

HepG2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human mtRNA polymerase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APOLMT antibody, MTRNAP antibody, MTRPOL antibody, h-mtRPOL antibody, POLRMT antibody, "DNA-directed RNA polymerase, mitochondrial antibody", polymerase (RNA) mitochondrial (DNA directed) antibody

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105137_WB_19011506_192.jpg

GTX105137 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
5% SDS PAGE
GTX105137 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105137_40408_IHC_19011506_248.jpg

GTX105137 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using mtRNA polymerase(GTX105137) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105137_39925_IFA_19011506_649.jpg

GTX105137 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep G2, using mtRNA polymerase(GTX105137) antibody at 1:500 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169