Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody [HL2012] (GTX637913) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody [HL2012] (GTX637913) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

mtTFA antibody [HL2012] detects mtTFA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
mtTFA stained by mtTFA antibody [HL2012] (GTX637913) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min