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mtTFA antibody, N-term

Anti-mtTFA antibody, N-term used in Western Blot (WB). GTX77852
Anti-mtTFA antibody, N-term used in Western Blot (WB). GTX77852
Anti-mtTFA antibody, N-term used in Western Blot (WB). GTX77852

Cat. No. GTX77852

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
50 μg ($409)
See all mtTFA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

bEND.3 , Hep3B , HepG2 , Jurkat , K562

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human mtTFA

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_424800

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor A, mitochondrial , MTDPS15 , MTTF1 , MTTFA , TCF6 , TCF6L1 , TCF6L2 , TCF6L3

Cellular Localization

Mitochondrion

Background

This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-mtTFA antibody, N-term used in Western Blot (WB). GTX77852

GTX77852 WB Image

WB analysis of Jurkat cells using GTX77852 mtTFA antibody at 0.2-1μg/ml.

Anti-mtTFA antibody, N-term used in Western Blot (WB). GTX77852

GTX77852 WB Image

WB analysis of bEND.3 cells using GTX77852 mtTFA antibody at 1:1000.
Lane 1 : bEND.3 cells (10μg)
Lane 2 : bEND.3 cells (20μg)

Anti-mtTFA antibody, N-term used in Western Blot (WB). GTX77852

GTX77852 WB Image

WB analysis of HepG2, Hep3B, and K562 cells using GTX77852 mtTFA antibody at 1:1000.
Lane :
1: HepG2 cell lysate
2: HepG2 cell lysate
3: Hep3B cell lysate
4: Hep3B cell lysate
5: K562 cell lysate
6: K562 cell lysate

REFERENCE

Application Reference

Signorile A et al. Biochim Biophys Acta 2014; 1843 (4) : 675-84 Regulation of the biogenesis of OXPHOS complexes in cell transition from replicating to quiescent state: involvement of PKA and effect of hydroxytyrosol.
Application : WB
Reactivity : Human
Antelman J et al. J Cell Physiol 2008; 217 (2) : 529-43 Expression of mitochondrial transcription factor A (TFAM) during porcine gametogenesis and preimplantation embryo development.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409