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mtTFA antibody

KO/KD Orthogonal
Anti-mtTFA antibody used in Immunoprecipitation (IP). GTX103231
Anti-mtTFA antibody used in Western Blot (WB). GTX103231
Anti-mtTFA antibody used in Western Blot (WB). GTX103231
Anti-mtTFA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103231
Anti-mtTFA antibody used in IHC (Paraffin sections) (IHC-P). GTX103231
Anti-mtTFA antibody used in IHC (Paraffin sections) (IHC-P). GTX103231
Anti-mtTFA antibody used in IHC (Paraffin sections) (IHC-P). GTX103231
Anti-mtTFA antibody used in Western Blot (WB). GTX103231

Cat. No. GTX103231

Reference 13
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all mtTFA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human mtTFA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176720

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor A, mitochondrial , MTDPS15 , MTTF1 , MTTFA , TCF6 , TCF6L1 , TCF6L2 , TCF6L3

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-mtTFA antibody used in Immunoprecipitation (IP). GTX103231

GTX103231 IP Image

mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 μg of mtTFA antibody (GTX103231) 15% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody (GTX103231) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-mtTFA antibody used in Western Blot (WB). GTX103231

GTX103231 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX103231) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-mtTFA antibody used in Western Blot (WB). GTX103231

GTX103231 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX103231) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mtTFA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103231

GTX103231 ICC/IF Image

mtTFA antibody detects mtTFA protein at mito by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mtTFA stained by mtTFA antibody (GTX103231) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-mtTFA antibody used in IHC (Paraffin sections) (IHC-P). GTX103231

GTX103231 IHC-P Image

mtTFA antibody detects mtTFA protein at cytoplasm in human esophagus cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus cancer.
mtTFA antibody (GTX103231) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mtTFA antibody used in IHC (Paraffin sections) (IHC-P). GTX103231

GTX103231 IHC-P Image

mtTFA antibody detects mtTFA protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
mtTFA antibody (GTX103231) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mtTFA antibody used in IHC (Paraffin sections) (IHC-P). GTX103231

GTX103231 IHC-P Image

mtTFA antibody detects mtTFA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
mtTFA stained by mtTFA antibody (GTX103231) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mtTFA antibody used in Western Blot (WB). GTX103231

GTX103231 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX103231) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zecchini V et al. Nature 2023; 615 (7952) : 499-506 Fumarate induces vesicular release of mtDNA to drive innate immunity.
Application : WB
Reactivity : Mouse
Rey T et al. Nucleic Acids Res 2023; 51 (21) : e107 mtFociCounter for automated single-cell mitochondrial nucleoid quantification and reproducible foci analysis.
David Molina-Granada et al. Commun Biol 2022; 5 (1) : 620 Most mitochondrial dGTP is tightly bound to respiratory complex I through the NDUFA10 subunit.
Application : WB
Reactivity : Mouse
Takuro Toda et al. Commun Biol 2022; 5 (1) : 453 Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria.
Application : WB
Armstrong N et al. Cell Rep 2022; 38 (9) : 110453 SDHB knockout and succinate accumulation are insufficient for tumorigenesis but dual SDHB/NF1 loss yields SDHx-like pheochromocytomas.
Application : ICC/IF
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

mtTFA antibody Cat. No. GTX103231

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-mtTFA antibody used in Western Blot (WB). GTX103231
Date : Anonymous submitted on 06-Nov-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 8μg
Blocking : 5% Skim Milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Submit a Review
Application Reference
Zecchini V et al. Nature 2023; 615 (7952):499-506 Fumarate induces vesicular release of mtDNA to drive innate immunity.
Application : WB
Reactivity : Mouse
Rey T et al. Nucleic Acids Res 2023; 51 (21):e107 mtFociCounter for automated single-cell mitochondrial nucleoid quantification and reproducible foci analysis.
David Molina-Granada et al. Commun Biol 2022; 5 (1):620 Most mitochondrial dGTP is tightly bound to respiratory complex I through the NDUFA10 subunit.
Application : WB
Reactivity : Mouse
Takuro Toda et al. Commun Biol 2022; 5 (1):453 Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria.
Application : WB
Armstrong N et al. Cell Rep 2022; 38 (9):110453 SDHB knockout and succinate accumulation are insufficient for tumorigenesis but dual SDHB/NF1 loss yields SDHx-like pheochromocytomas.
Application : ICC/IF
Reactivity : Mouse
Vicente Roca-Agujetas et al. Mol Neurodegener 2021; 16 (1):15 Cholesterol alters mitophagy by impairing optineurin recruitment and lysosomal clearance in Alzheimer's disease.
Ai Sato et al. STAR Protoc 2021; 2 (2):100488 Immunofluorescence microscopy-based assessment of cytosolic DNA accumulation in mammalian cells.
Lam HYP et al. Parasitol Int 2021; 80:102231 Mitochondrial dynamics in Angiostrongylus cantonensis-infected mouse brain.
Application : WB
Reactivity : Mouse
Zhuo M et al. FEBS Open Bio 2020; 10 (5):789-801
Application : ICC/IF
Reactivity : Mouse
Kurundkar D et al. JCI Insight 2019; 4 (1) SIRT3 diminishes inflammation and mitigates endotoxin-induced acute lung injury.
Application : WB
Reactivity : Mouse
Zhuo M et al. Free Radic Biol Med 2018; 121:43362 neurotoxicity of * (ara-c) in dorsal root ganglion neurons originates from impediment of mtdna synthesis and compromise of mitochondrial function.
Application : ICC/IF
Reactivity : Mouse
Gorgun MF et al. Mol Neurobiol 2016; (Epub) * toxicity in dorsal root ganglion neurons is relieved by meclizine via diminution of mitochondrial compromise and improved clearance of dna damage.
Application : ICC/IF
Reactivity : Mouse
Kim SY et al. Cell Death Dis 2014; 5:e1105 Inhibition of cyclophilin D by cyclosporin A promotes retinal ganglion cell survival by preventing mitochondrial alteration in ischemic injury.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169