GeneTex
United States (US)

mtTFA antibody

KO/KD
GTX103231_40877_20150410_IFA_19011506_895.jpg
GTX103231_40877_IP_19011506_920.jpg
GTX103231_40877_20150420_IHC_2_19011506_953.jpg
GTX103231_40877_20150420_IHC_19011506_479.jpg
GTX103231_40877_20180907_WB_shRNA_watermark_19011506_611.jpg
GTX103231_40877_WB_19011506_939.jpg

Cat No. GTX103231

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human mtTFA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MtTF1 antibody, TCF6 antibody, TCF6L1 antibody, TCF6L2 antibody, TCF6L3 antibody, mtTFA antibody, TFAM antibody, Transcription factor 6-like 2 (mitochondrial transcription factor) antibody, transcription factor A, mitochondrial antibody, mitochondrial transcription factor 1 antibody, transcription factor 6 antibody, mitochondrial transcription factor A antibody, TCF-6 antibody

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103231_40877_20150410_IFA_19011506_895.jpg

GTX103231 ICC/IF Image

mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mtTFA protein stained by mtTFA antibody (GTX103231) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX103231_40877_IP_19011506_920.jpg

GTX103231 IP Image

mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 μg of mtTFA antibody (GTX103231) 15% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody (GTX103231) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103231_40877_20150420_IHC_2_19011506_953.jpg

GTX103231 IHC-P Image

mtTFA antibody detects mtTFA protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
mtTFA antibody (GTX103231) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103231_40877_20150420_IHC_19011506_479.jpg

GTX103231 IHC-P Image

mtTFA antibody detects mtTFA protein at cytoplasm in human esophagus cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus cancer.
mtTFA antibody (GTX103231) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103231_40877_20180907_WB_shRNA_watermark_19011506_611.jpg

GTX103231 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX103231) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103231_40877_WB_19011506_939.jpg

GTX103231 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
12% SDS PAGE
GTX103231 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

mtTFA antibody Cat No. GTX103231

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-Nov-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 8μg
Blocking : 5% Skim Milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169