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mtTFA antibody

Anti-mtTFA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103231
Anti-mtTFA antibody used in Immunoprecipitation (IP). GTX103231
Anti-mtTFA antibody used in IHC (Paraffin sections) (IHC-P). GTX103231
Anti-mtTFA antibody used in IHC (Paraffin sections) (IHC-P). GTX103231
Anti-mtTFA antibody used in Western Blot (WB). GTX103231
Anti-mtTFA antibody used in Western Blot (WB). GTX103231

Cat No. GTX103231

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human mtTFA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

transcription factor A, mitochondrial , MTDPS15 , MTTF1 , MTTFA , TCF6 , TCF6L1 , TCF6L2 , TCF6L3

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-mtTFA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103231

GTX103231 ICC/IF Image

mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mtTFA protein stained by mtTFA antibody (GTX103231) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-mtTFA antibody used in Immunoprecipitation (IP). GTX103231

GTX103231 IP Image

mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 μg of mtTFA antibody (GTX103231) 15% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody (GTX103231) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-mtTFA antibody used in IHC (Paraffin sections) (IHC-P). GTX103231

GTX103231 IHC-P Image

mtTFA antibody detects mtTFA protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
mtTFA antibody (GTX103231) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mtTFA antibody used in IHC (Paraffin sections) (IHC-P). GTX103231

GTX103231 IHC-P Image

mtTFA antibody detects mtTFA protein at cytoplasm in human esophagus cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus cancer.
mtTFA antibody (GTX103231) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mtTFA antibody used in Western Blot (WB). GTX103231

GTX103231 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX103231) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mtTFA antibody used in Western Blot (WB). GTX103231

GTX103231 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
12% SDS PAGE
GTX103231 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

mtTFA antibody Cat No. GTX103231

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-Nov-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 8μg
Blocking : 5% Skim Milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169