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n-Myc antibody, C-term

Orthogonal
Anti-n-Myc antibody, C-term used in Western Blot (WB). GTX81475
Anti-n-Myc antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81475
Anti-n-Myc antibody, C-term used in Western Blot (WB). GTX81475

Cat. No. GTX81475

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
400 μl ($399)
See all n-Myc products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 322-351 amino acids from the C-terminal region of human MYCN.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_11172462

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYCN proto-oncogene, bHLH transcription factor , MODED , N-myc , NMYC , ODED , bHLHe37

Cellular Localization

Nucleus

Background

This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES

Anti-n-Myc antibody, C-term used in Western Blot (WB). GTX81475

GTX81475 WB Image

WB analysis of CCRF-CEM whole cell lysate using GTX81475 n-Myc antibody, C-term.
Loading : 20 μg per lane
Dilution : 1:1000

Anti-n-Myc antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81475

GTX81475 IHC-P Image

IHC-P analysis of human brain tissue using GTX81475 n-Myc antibody, C-term.

Anti-n-Myc antibody, C-term used in Western Blot (WB). GTX81475

GTX81475 WB Image

Orthogonal

The data was published in the journal Mol Cancer in 2017. PMID: 28327152

REFERENCE

Application Reference

Mastropasqua F et al. Mol Cancer 2017; 16 (1) : 67 TRIM8 restores p53 tumour suppressor function by blunting N-MYC activity in chemo-resistant tumours.
Application : WB
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399