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n-Myc antibody [NMYC-1]

Anti-n-Myc antibody [NMYC-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20057
Anti-n-Myc antibody [NMYC-1] used in Western Blot (WB). GTX20057
Anti-n-Myc antibody [NMYC-1] used in Western Blot (WB). GTX20057

Cat. No. GTX20057

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 NMYC-1

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS, IP, ChIP assay, Gel supershift assays, MS

Reactivity

 Human, Mouse
Package
100 μg ($419)
See all n-Myc products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:100
FACS Assay dependent
IP 1:10 - 1:100
ChIP assay 1:10 - 1:500
Gel supershift assays 1:1 - 1:100
MS Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Product Note

This does not react with c-Myc or L-Myc.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide (SPYVESEDAPPQKC) conjugated to KLH, corresponding to amino acids 327-339 of human n-Myc. [UniProt# P04198]

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYCN proto-oncogene, bHLH transcription factor , MODED , N-myc , NMYC , ODED , bHLHe37

Cellular Localization

Nucleus

Background

This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-n-Myc antibody [NMYC-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20057

GTX20057 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX20057 n-Myc antibody [NMYC-1].
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:50

Anti-n-Myc antibody [NMYC-1] used in Western Blot (WB). GTX20057

GTX20057 WB Image

WB analysis of human heart tissue lysate using GTX20057 n-Myc antibody [NMYC-1].

Anti-n-Myc antibody [NMYC-1] used in Western Blot (WB). GTX20057

GTX20057 WB Image

WB analysis of IMR-32 cell lysate using GTX20057 n-Myc antibody [NMYC-1].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419