GeneTex
  • Country / Location Selection

United States (US)

nNOS antibody, C-term

Anti-nNOS antibody, C-term used in Flow cytometry (FACS). GTX89962
Anti-nNOS antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89962
Anti-nNOS antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89962
Anti-nNOS antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89962

Cat No. GTX89962

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P, IHC-Fr, IHC-Wm, FACS, IHC

Reactivity

Human, Mouse
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P 5μg/ml
IHC-Fr Assay dependent
IHC-Wm Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89962-PEP).

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ESKKDTDEVFSS, from the C Terminus of the protein sequence according to NP_000611.1; NP_001191147.1; NP_001191142.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10725945

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nitric oxide synthase 1 , IHPS1 , N-NOS , NC-NOS , NOS , bNOS , nNOS

Background

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-nNOS antibody, C-term used in Flow cytometry (FACS). GTX89962

GTX89962 FACS Image

FACS analysis of paraformaldehyde fixed Kelly cells using GTX89962 nNOS antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Anti-nNOS antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89962

GTX89962 ICC/IF Image

ICC/IF analysis of paraformaldehyde fixed HeLa cells using GTX89962 nNOS antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml

Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml

Anti-nNOS antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89962

GTX89962 IHC-Fr Image

IHC-Fr analysis of perfusion-fixed (4% PFA) mouse caudate-putamen using GTX89962 nNOS antibody, C-term.

Anti-nNOS antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89962

GTX89962 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX89962 nNOS antibody, C-term.
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

REFERENCE

REVIEW

There are currently no reviews for nNOS antibody, C-term (GTX89962). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 379