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nNOS antibody, C-term

Anti-nNOS antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89962
Anti-nNOS antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89962
Anti-nNOS antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89962
Anti-nNOS antibody, C-term used in Flow cytometry (FACS). GTX89962

Cat. No. GTX89962

Reference 5

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr, IHC-Wm, FACS, IHC

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all nNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 5μg/ml
IHC-Fr Assay dependent
IHC-Wm Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89962-PEP).

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ESKKDTDEVFSS, from the C Terminus of the protein sequence according to NP_000611.1; NP_001191147.1; NP_001191142.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10725945

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 1 , IHPS1 , N-NOS , NC-NOS , NOS , bNOS , nNOS

Background

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-nNOS antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89962

GTX89962 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX89962 nNOS antibody, C-term.
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-nNOS antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89962

GTX89962 IHC-Fr Image

IHC-Fr analysis of perfusion-fixed (4% PFA) mouse caudate-putamen using GTX89962 nNOS antibody, C-term.

Anti-nNOS antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89962

GTX89962 ICC/IF Image

ICC/IF analysis of paraformaldehyde fixed HeLa cells using GTX89962 nNOS antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml

Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml

Anti-nNOS antibody, C-term used in Flow cytometry (FACS). GTX89962

GTX89962 FACS Image

FACS analysis of paraformaldehyde fixed Kelly cells using GTX89962 nNOS antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

REFERENCE

Application Reference

Zaninelli TH et al. Pharmaceuticals (Basel) 2023; 16 (3) Kaurenoic Acid Reduces Ongoing Chronic Constriction Injury-Induced Neuropathic Pain: Nitric Oxide Silencing of Dorsal Root Ganglia Neurons.
Application : IHC-Fr
Aktar R et al. Gut Microbes 2020; 11 (6) : 1745-1757 Human resident gut microbe Bacteroides thetaiotaomicron regulates colonic neuronal innervation and neurogenic function.
DiCello JJ et al. Cell Mol Gastroenterol Hepatol 2019; (Epub) Mu and Delta Opioid Receptors Are Coexpressed and Functionally Interact in the Enteric Nervous System of the Mouse Colon.
Application : IHC
Reactivity : Mouse
DiCello JJ et al. Am J Physiol Gastrointest Liver Physiol 2019; (Epub) Clathrin and GRK2/3 inhibitors block delta opioid receptor internalization in myenteric neurons and inhibit neuromuscular transmission in the mouse colon.
Application : IHC-P
Reactivity : Mouse
DiCello JJ et al. Cell Mol Life Sci 2019; Agonist-dependent development of delta opioid receptor tolerance in the colon.
Application : IHC-Wm
Reactivity : Mouse
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399