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nNOS antibody

Anti-nNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX133403
Anti-nNOS antibody used in IHC (Frozen sections) (IHC-Fr). GTX133403
Anti-nNOS antibody used in Western Blot (WB). GTX133403
Anti-nNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX133403
Anti-nNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133403
Anti-nNOS antibody used in IHC (Frozen sections) (IHC-Fr). GTX133403

Cat. No. GTX133403

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all nNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Rat nNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886968

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 1 , bNOS

Cellular Localization

Cell membrane , sarcolemma; Peripheral membrane protein , Cell projection , dendritic spine

Background

enzyme that catalyzes the production of nitric oxide [RGD]

Database

Research Area

DATA IMAGES

Anti-nNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX133403

GTX133403 IHC-P Image

nNOS antibody detects nNOS protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
nNOS antibody (GTX133403) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-nNOS antibody used in IHC (Frozen sections) (IHC-Fr). GTX133403

GTX133403 IHC-Fr Image

nNOS antibody detects nNOS protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: nNOS protein stained by nNOS antibody (GTX133403) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-nNOS antibody used in Western Blot (WB). GTX133403

GTX133403 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with nNOS antibody (GTX133403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-nNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX133403

GTX133403 IHC-P Image

nNOS antibody detects nNOS protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
nNOS antibody (GTX133403) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-nNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133403

GTX133403 ICC/IF Image

nNOS antibody detects nNOS protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: nNOS stained by nNOS antibody (GTX133403) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-nNOS antibody used in IHC (Frozen sections) (IHC-Fr). GTX133403

GTX133403 IHC-Fr Image

The data was published in the 2022 in Front Neurosci. PMID: 35242008

REFERENCE

Application Reference

Fan M et al. Cell Mol Gastroenterol Hepatol 2023; 15 (2) : 511-531 BMSCs Promote Differentiation of Enteric Neural Precursor Cells to Maintain Neuronal Homeostasis in Mice With Enteric Nerve Injury.
Application : ICC/IF
Reactivity : Mouse
De-Fong Huang et al. 2022; 119 (33) Neuronal splicing regulator RBFOX3 mediates seizures via regulating Vamp1 expression preferentially in NPY-expressing GABAergic neurons.
Application : IHC-Fr
Reactivity : Mouse
Xuehui Fan et al. Front Neurosci 2022; 16 : 838621 Inhibiting Sphingosine 1-Phosphate Receptor Subtype 3 Attenuates Brain Damage During Ischemia-Reperfusion Injury by Regulating nNOS/NO and Oxidative Stress.
Application : IHC-Fr
Reactivity : Mouse
Jarret A et al. Cell 2020; 180 (1) : 50-63.e12 Enteric Nervous System-Derived IL-18 Orchestrates Mucosal Barrier Immunity.
Application : IHC-Fr
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169