APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Human, Rabbit, Dog, Japanese Medaka(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.79 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human nNOS. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886970
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nitric oxide synthase 1 , IHPS1 , N-NOS , NC-NOS , NOS , bNOS , nNOS
Cellular Localization
Cell membrane , sarcolemma; Peripheral membrane protein , Cell projection , dendritic spine
Background
Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter; it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. NO is also responsible for endothelium-derived relaxing factor activity regulating blood pressure. In macrophages, NO mediates tumoricidal and bactericidal actions, as indicated by the fact that inhibitors of NO synthase (NOS) block these effects. Neuronal NOS and macrophage NOS (MIM 163730) are distinct isoforms (Lowenstein et al., 1992 [PubMed 1379716]). Both the neuronal and the macrophage forms are unusual among oxidative enzymes in requiring several electron donors: FAD (see MIM 610595), flavin mononucleotide (FMN), NADPH, and tetrahydrobiopterin.[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX133407 IHC-P Image
nNOS antibody detects nNOS protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
nNOS antibody (GTX133407) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX133407 IHC-P Image
nNOS antibody detects nNOS protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
nNOS antibody (GTX133407) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX133407 ICC/IF Image
nNOS antibody detects nNOS protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: nNOS stained by nNOS antibody (GTX133407) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX133407 IHC-Fr Image
nNOS antibody detects nNOS protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: nNOS protein stained by nNOS antibody (GTX133407) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX133407 WB Image
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with nNOS antibody (GTX133407) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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