IHC-P analysis of rat brain tissue using GTX82905 nNOS antibody.

ICC/IF analysis of SH-SY5Y cells using GTX82905 nNOS antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

WB analysis of 40 μg of mouse (Lane 1) and rat brain (Lane 2) tissue lysate using GTX82905 nNOS antibody.
Dilution : 1:1000

IHC-P analysis of mouse skeletal muscle tissue using GTX82905 nNOS antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:400

IHC-P analysis of rat cerebellum tissue using GTX82905 nNOS antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200