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nm23-H1 antibody [4B2]

Anti-nm23-H1 antibody [4B2] used in IHC (Paraffin sections) (IHC-P). GTX83325
Anti-nm23-H1 antibody [4B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83325
Anti-nm23-H1 antibody [4B2] used in Flow cytometry (FACS). GTX83325
Anti-nm23-H1 antibody [4B2] used in Western Blot (WB). GTX83325

Cat. No. GTX83325

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all nm23-H1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NME1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NME/NM23 nucleoside diphosphate kinase 1 , AWD , GAAD , NB , NBS , NDKA , NDPK-A , NDPKA , NM23 , NM23-H1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-nm23-H1 antibody [4B2] used in IHC (Paraffin sections) (IHC-P). GTX83325

GTX83325 IHC-P Image

IHC-P analysis of human Placenta tissue using GTX83325 nm23-H1 antibody [4B2].

Anti-nm23-H1 antibody [4B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83325

GTX83325 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX83325 nm23-H1 antibody [4B2].
Green : nm23-H1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-nm23-H1 antibody [4B2] used in Flow cytometry (FACS). GTX83325

GTX83325 FACS Image

FACS analysis of Jurkat cells using GTX83325 nm23-H1 antibody [4B2].
Green : nm23-H1
Purple : negative control

Anti-nm23-H1 antibody [4B2] used in Western Blot (WB). GTX83325

GTX83325 WB Image

WB analysis of nm23-H1(AA: 27-177)-hIgGFc transfected HEK293 cell lysate using GTX83325 nm23-H1 antibody [4B2].

REFERENCE

Application Reference

Kim D et al. J Clin Med 2020; 9 (10) Negative Effect of Reduced NME1 Expression on Recurrence-Free Survival in Early Stage Non-Small Cell Lung Cancer.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399