APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Based on internal testing result, this antibody does not cross react with human NME2, human NME3, and human NME4.
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT5F4 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NME3 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NME/NM23 nucleoside diphosphate kinase 1 , AWD , GAAD , NB , NBS , NDKA , NDPKA , NM23 , NM23H1 , NME1 , nm23-H1
Cellular Localization
Cytoplasm,Nucleus
Background
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-nm23-H1 antibody [AT5F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57703](/templates/default/js/lightbox/images/loading.gif "Anti-nm23-H1 antibody [AT5F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57703")
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GTX57703 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57703 nm23-H1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57703 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57703 nm23-H1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57703 WB Image
WB analysis of various samples using GTX57703 nm23-H1 antibody.
Lane 1 : Recombinant Human NME1 protein
Lane 2 : Recombinant Human NME2 protein
Lane 3 : Recombinant Human NME3 protein
Lane 4 : Recombinant Human NME4 protein
Loading : 50 ng
Dilution : 1:1000
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GTX57703 WB Image
WB analysis of various samples using GTX57703 nm23-H1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : MCF-7 whole cell lysate
Lane 6 : PC-3 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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REFERENCE
There are currently no references for nm23-H1 antibody [AT5F4] (GTX57703). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for nm23-H1 antibody [AT5F4] (GTX57703). Be the first to share your experience with this product.
![Anti-nm23-H1 antibody [AT5F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57703](/upload/website/prouct_img/small/GTX57703/GTX57703_20200204_IF1_w_23061123_368.webp "Anti-nm23-H1 antibody [AT5F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57703")
![Anti-nm23-H1 antibody [AT5F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57703](/upload/website/prouct_img/small/GTX57703/GTX57703_20200204_IF2_w_23061123_443.webp "Anti-nm23-H1 antibody [AT5F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57703")
![Anti-nm23-H1 antibody [AT5F4] used in Western Blot (WB). GTX57703](/upload/website/prouct_img/small/GTX57703/GTX57703_20200204_WB2_w_23061123_693.webp "Anti-nm23-H1 antibody [AT5F4] used in Western Blot (WB). GTX57703")
![Anti-nm23-H1 antibody [AT5F4] used in Western Blot (WB). GTX57703](/upload/website/prouct_img/small/GTX57703/GTX57703_20200204_WB1_w_23061123_182.webp "Anti-nm23-H1 antibody [AT5F4] used in Western Blot (WB). GTX57703")
![Anti-nm23-H1 antibody [AT5F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57703](/upload/website/prouct_img/normal/GTX57703/GTX57703_20200204_IF1_w_23061123_368.webp "Anti-nm23-H1 antibody [AT5F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57703")
![Anti-nm23-H1 antibody [AT5F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57703](/upload/website/prouct_img/normal/GTX57703/GTX57703_20200204_IF2_w_23061123_443.webp "Anti-nm23-H1 antibody [AT5F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57703")
![Anti-nm23-H1 antibody [AT5F4] used in Western Blot (WB). GTX57703](/upload/website/prouct_img/normal/GTX57703/GTX57703_20200204_WB2_w_23061123_693.webp "Anti-nm23-H1 antibody [AT5F4] used in Western Blot (WB). GTX57703")
![Anti-nm23-H1 antibody [AT5F4] used in Western Blot (WB). GTX57703](/upload/website/prouct_img/normal/GTX57703/GTX57703_20200204_WB1_w_23061123_182.webp "Anti-nm23-H1 antibody [AT5F4] used in Western Blot (WB). GTX57703")