APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , Raji
Predict Reactivity
Mouse, Rat, Rabbit, Dog, Guinea pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.98 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human nm23-H1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951004
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NME/NM23 nucleoside diphosphate kinase 1 , AWD , GAAD , NB , NBS , NDKA , NDPK-A , NDPKA , NM23 , NM23-H1
Cellular Localization
Cytoplasm , Nucleus
Background
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-nm23-H1 antibody [C1C3] used in Western Blot (WB). GTX108325](/templates/default/js/lightbox/images/loading.gif "Anti-nm23-H1 antibody [C1C3] used in Western Blot (WB). GTX108325")
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GTX108325 WB Image
Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody [C1C3] (GTX108325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX108325 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using nm23-H1(GTX108325) antibody at 1:200 dilution.
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GTX108325 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody (GTX108325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX108325 IHC-P Image
nm23-H1 antibody [C1C3] detects nm23-H1 protein at cytosol on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
nm23-H1 antibody [C1C3] (GTX108325) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for nm23-H1 antibody [C1C3] (GTX108325). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for nm23-H1 antibody [C1C3] (GTX108325). Be the first to share your experience with this product.
![Anti-nm23-H1 antibody [C1C3] used in Western Blot (WB). GTX108325](/upload/website/prouct_img/small/GTX108325/GTX108325_39834_20181109_WB_KO_watermark_w_23060120_856.webp "Anti-nm23-H1 antibody [C1C3] used in Western Blot (WB). GTX108325")
![Anti-nm23-H1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108325](/upload/website/prouct_img/small/GTX108325/GTX108325_IFA_w_23060120_263.webp "Anti-nm23-H1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108325")
![Anti-nm23-H1 antibody [C1C3] used in Western Blot (WB). GTX108325](/upload/website/prouct_img/small/GTX108325/GTX108325_39834_20181109_WB_w_23060120_430.webp "Anti-nm23-H1 antibody [C1C3] used in Western Blot (WB). GTX108325")
![Anti-nm23-H1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108325](/upload/website/prouct_img/small/GTX108325/GTX108325_39834_IHC_w_23060120_852.webp "Anti-nm23-H1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108325")
![Anti-nm23-H1 antibody [C1C3] used in Western Blot (WB). GTX108325](/upload/website/prouct_img/normal/GTX108325/GTX108325_39834_20181109_WB_KO_watermark_w_23060120_856.webp "Anti-nm23-H1 antibody [C1C3] used in Western Blot (WB). GTX108325")
![Anti-nm23-H1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108325](/upload/website/prouct_img/normal/GTX108325/GTX108325_IFA_w_23060120_263.webp "Anti-nm23-H1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108325")
![Anti-nm23-H1 antibody [C1C3] used in Western Blot (WB). GTX108325](/upload/website/prouct_img/normal/GTX108325/GTX108325_39834_20181109_WB_w_23060120_430.webp "Anti-nm23-H1 antibody [C1C3] used in Western Blot (WB). GTX108325")
![Anti-nm23-H1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108325](/upload/website/prouct_img/normal/GTX108325/GTX108325_39834_IHC_w_23060120_852.webp "Anti-nm23-H1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108325")