GeneTex
United States (US)

nm23-H1 antibody [C1C3]

KO/KD
GTX108325_39834_20181109_WB_KO_watermark_19011519_492.jpg
GTX108325_39834_20181109_WB_19011519_657.jpg
GTX108325_IFA_19011507_201.jpg
GTX108325_39834_IHC_19011507_582.jpg
GTX108325_WB_19011507_926.jpg

Cat No. GTX108325

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

293T , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human nm23-H1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nme/Nm23 Nucleoside Diphosphate Kinase 1,Awd,Gaad,Nb,Nbs,Ndka,Ndpk-A,Ndpka,Nm23,Nm23-H1,Nme1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108325_39834_20181109_WB_KO_watermark_19011519_492.jpg

GTX108325 WB Image

KO/KD

Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody [C1C3] (GTX108325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX108325_39834_20181109_WB_19011519_657.jpg

GTX108325 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody (GTX108325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108325_IFA_19011507_201.jpg

GTX108325 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using nm23-H1(GTX108325) antibody at 1:200 dilution.

GTX108325_39834_IHC_19011507_582.jpg

GTX108325 IHC-P Image

nm23-H1 antibody [C1C3] detects nm23-H1 protein at cytosol on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
nm23-H1 antibody [C1C3] (GTX108325) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108325_WB_19011507_926.jpg

GTX108325 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:Raji(GTX27908)
12% SDS PAGE
GTX108325 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169