Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody [N1C3] (GTX112988) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX112988 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
12% SDS PAGE
GTX112988 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using nm23-H1(GTX112988) antibody at 1:100 dilution.