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nm23-H1 antibody

Anti-nm23-H1 antibody used in Western Blot (WB). GTX55727
Anti-nm23-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55727
Anti-nm23-H1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55727
Anti-nm23-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55727
Anti-nm23-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55727
Anti-nm23-H1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55727

Cat. No. GTX55727

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all nm23-H1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human NME1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NME/NM23 nucleoside diphosphate kinase 1 , AWD , GAAD , NB , NBS , NDKA , NDPK-A , NDPKA , NM23 , NM23-H1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-nm23-H1 antibody used in Western Blot (WB). GTX55727

GTX55727 WB Image

WB analysis of various sample lysates using GTX55727 nm23-H1 antibody.
Loading : 25μg per lane

Anti-nm23-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55727

GTX55727 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX55727 nm23-H1 antibody.
Dilution : 1:100

Anti-nm23-H1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55727

GTX55727 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX55727 nm23-H1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-nm23-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55727

GTX55727 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX55727 nm23-H1 antibody.
Dilution : 1:100

Anti-nm23-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55727

GTX55727 IHC-P Image

IHC-P analysis of human colon tissue using GTX55727 nm23-H1 antibody.
Dilution : 1:100

Anti-nm23-H1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55727

GTX55727 ICC/IF Image

ICC/IF analysis of C6 cells using GTX55727 nm23-H1 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399