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nm23-H2 antibody

Anti-nm23-H2 antibody used in Western Blot (WB). GTX101706
Anti-nm23-H2 antibody used in Western Blot (WB). GTX101706
Anti-nm23-H2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101706
Anti-nm23-H2 antibody used in Western Blot (WB). GTX101706

Cat. No. GTX101706

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all nm23-H2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , Mouse brain

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human nm23-H2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241112

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NME/NM23 nucleoside diphosphate kinase 2 , NDKB , NDPK-B , NDPKB , NM23-H2 , NM23B , PUF

Cellular Localization

Cytoplasm , Nucleus

Background

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-nm23-H2 antibody used in Western Blot (WB). GTX101706

GTX101706 WB Image

nm23-H2 antibody detects nm23-H2 protein by western blot analysis.
A. 30 μg A549 whole cell extract
B. 30 μg H1299 whole cell extract
C. 30 μg HCT116 whole cell extract
12 % SDS-PAGE
nm23-H2 antibody (GTX101706) dilution: 1:1000

Anti-nm23-H2 antibody used in Western Blot (WB). GTX101706

GTX101706 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX101706 diluted at 1:1000

Anti-nm23-H2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101706

GTX101706 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish muscle, using nm23-H2 antibody (GTX101706) at 1:300 dilution.

Anti-nm23-H2 antibody used in Western Blot (WB). GTX101706

GTX101706 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
B: zebrafish heart
15% SDS PAGE
GTX101706 diluted at 1:1000

REFERENCE

Application Reference

Chang SJ et al. Arch Biochem Biophys 2018; 647 : 10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169