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nm23-H2 antibody

Anti-nm23-H2 antibody used in Western Blot (WB). GTX101706
Anti-nm23-H2 antibody used in Western Blot (WB). GTX101706

Cat No. GTX101706

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , mouse brain

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human nm23-H2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NME/NM23 nucleoside diphosphate kinase 2 , NDKB , NDPK-B , NDPKB , NM23-H2 , NM23B , PUF

Cellular Localization

Cytoplasm , Nucleus

Background

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-nm23-H2 antibody used in Western Blot (WB). GTX101706

GTX101706 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX101706 diluted at 1:1000

Anti-nm23-H2 antibody used in Western Blot (WB). GTX101706

GTX101706 WB Image

nm23-H2 antibody detects nm23-H2 protein by western blot analysis.
A. 30 μg A549 whole cell extract
B. 30 μg H1299 whole cell extract
C. 30 μg HCT116 whole cell extract
12 % SDS-PAGE
nm23-H2 antibody (GTX101706) dilution: 1:1000

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169