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nm23-H2 antibody

Anti-nm23-H2 antibody used in Western Blot (WB). GTX66869

Cat No. GTX66869

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 87-130 of Human NM23-H2.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NME/NM23 nucleoside diphosphate kinase 2 , NDKB , NDPK-B , NDPKB , NM23-H2 , NM23B , PUF

Cellular Localization

Cytoplasm,Nucleus

Background

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-nm23-H2 antibody used in Western Blot (WB). GTX66869

GTX66869 WB Image

WB analysis of various samples using GTX66869 nm23-H2 antibody.
Lane1 : MCF-7 whole cell lysate(40ug)
Lane2 : HEK293T whole cell lysate(40ug)
Lane3 : Hela whole cell lysate(40ug)
Lane4 : H9C2 whole cell lysate(40ug)
Lane5 : BV2 whole cell lysate(40ug)
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf