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p120 Catenin (phospho Ser268) antibody

KO/KD Orthogonal
Anti-p120 Catenin (phospho Ser268) antibody used in Western Blot (WB). GTX130428
Anti-p120 Catenin (phospho Ser268) antibody used in Western Blot (WB). GTX130428
Anti-p120 Catenin (phospho Ser268) antibody used in Western Blot (WB). GTX130428

Cat. No. GTX130428

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all p120 Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

A431 , NIH-3T3 , C2C12

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser268 of human p120 Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886264

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin delta 1 , BCDS2 , CAS , CTNND , P120CAS , P120CTN , p120 , p120(CAS) , p120(CTN)

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-p120 Catenin (phospho Ser268) antibody used in Western Blot (WB). GTX130428

GTX130428 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p120 Catenin (phospho Ser268) antibody (GTX130428) diluted at 1:3000.

Anti-p120 Catenin (phospho Ser268) antibody used in Western Blot (WB). GTX130428

GTX130428 WB Image

Orthogonal

p120 Catenin (phospho Ser268) antibody detects p120 Catenin (phospho Ser268) protein by western blot analysis. Un-treated (-) and treated (+, CIP treatment) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p120 Catenin (phospho Ser268) antibody (GTX130428) diluted at 1:1000. (CIP: Calf-intestinal alkaline phosphatase)

Anti-p120 Catenin (phospho Ser268) antibody used in Western Blot (WB). GTX130428

GTX130428 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p120 Catenin (phospho Ser268) antibody (GTX130428) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169