APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
H1299
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human p130Cas. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888375
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCAR1 scaffold protein, Cas family member , CAS , CAS1 , CASS1 , CRKAS , P130Cas
Cellular Localization
Cell junction , focal adhesion , Cytoplasm
Background
BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX633771 IHC-P Image
p130Cas antibody [GT331] detects p130Cas protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. p130Cas antibody [GT331] (GTX633771) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633771 IHC-Fr Image
p130Cas antibody [GT331] detects p130Cas protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: p130Cas protein stained by p130Cas antibody [GT331] (GTX633771) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX633771 IHC-P Image
p130Cas antibody [GT331] detects p130Cas protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. p130Cas antibody [GT331] (GTX633771) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633771 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas antibody [GT331] (GTX633771) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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