GeneTex
United States (US)

p130Cas antibody [N2C2], Internal

KO/KD
GTX100605_39694_20170518_WB_shRNA_watermark_19011506_399.jpg
GTX100605_41808_20171024_IHC-P_R_19011506_499.jpg
GTX100605_WB_19011506_604.jpg
GTX100605_41808_20170831_IHC-Fr_M_19011506_634.jpg
GTX100605_41808_20171024_IHC-P_M_19011506_747.jpg
GTX100605_39694_IFA_19011506_703.jpg
GTX100605_WB_M_19011506_163.jpg
GTX100605_39694_20141219_IHC_19011506_637.jpg

Cat No. GTX100605

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

H1299 , NIH-3T3 , 293T , p130Cas shRNA-transfected 293T

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.379mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCAR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CAS antibody, CAS1 antibody, CASS1 antibody, CRKAS antibody, FLJ12176 antibody, FLJ45059 antibody, P130Cas antibody, BCAR1 antibody, Crk-associated substrate p130Cas antibody, breast cancer anti-estrogen resistance protein 1 antibody, Cas scaffolding protein family member 1 antibody, breast cancer anti-estrogen resistance 1 antibody

Cellular Localization

Cell junction , focal adhesion , Cytoplasm

Background

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX100605_39694_20170518_WB_shRNA_watermark_19011506_399.jpg

GTX100605 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100605_41808_20171024_IHC-P_R_19011506_499.jpg

GTX100605 IHC-P Image

p130Cas antibody [N2C2], Internal detects p130Cas protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100605_WB_19011506_604.jpg

GTX100605 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX100605 diluted at 1:1000

GTX100605_41808_20170831_IHC-Fr_M_19011506_634.jpg

GTX100605 IHC-Fr Image

p130Cas antibody [N2C2], Internal detects p130Cas protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: p130Cas protein stained by p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX100605_41808_20171024_IHC-P_M_19011506_747.jpg

GTX100605 IHC-P Image

p130Cas antibody [N2C2], Internal detects p130Cas protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100605_39694_IFA_19011506_703.jpg

GTX100605 ICC/IF Image

BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BCAR1 protein stained by BCAR1 antibody [N2C2], Internal (GTX100605) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100605_WB_M_19011506_163.jpg

GTX100605 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX100605 diluted at 1:1000

GTX100605_39694_20141219_IHC_19011506_637.jpg

GTX100605 IHC-P Image

BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
BCAR1 antibody [N2C2], Internal (GTX100605) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169