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p130Cas (phospho Tyr165) antibody

Anti-p130Cas (phospho Tyr165) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132160
Anti-p130Cas (phospho Tyr165) antibody used in Western Blot (WB). GTX132160
Anti-p130Cas (phospho Tyr165) antibody used in Western Blot (WB). GTX132160
Anti-p130Cas (phospho Tyr165) antibody used in Western Blot (WB). GTX132160

Cat No. GTX132160

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , C2C12 , Rat2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human p130Cas (phospho Tyr165). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BCAR1 scaffold protein, Cas family member,CAS,CAS1,CASS1,CRKAS,P130Cas

Cellular Localization

Cell junction , focal adhesion , Cytoplasm

Background

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-p130Cas (phospho Tyr165) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132160

GTX132160 ICC/IF Image

p130Cas (phospho Tyr165) antibody detects p130Cas (phospho Tyr165) protein at cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: p130Cas (phospho Tyr165) protein stained by p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-p130Cas (phospho Tyr165) antibody used in Western Blot (WB). GTX132160

GTX132160 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)

Anti-p130Cas (phospho Tyr165) antibody used in Western Blot (WB). GTX132160

GTX132160 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p130Cas (phospho Tyr165) antibody used in Western Blot (WB). GTX132160

GTX132160 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW

p130Cas (phospho Tyr165) antibody Cat No. GTX132160

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Sep-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : ARPE-19 cells
Blocking : 3% BSA and 0.02% Triton-X100 in PBS, 25°C, 1Hr
Primary Antibody : 1:50, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169