GeneTex
United States (US)

p130Cas (phospho Tyr249) antibody

GTX32286_20190131_ICCIF_19013101_937.jpg
GTX32286_20190131_WB_19013102_583.jpg

Cat No. GTX32286

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

93 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of p130Cas. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcar1, Cas Family Scaffold Protein,Cas,Cas1,Cass1,Crkas,P130Cas,Bcar1

Cellular Localization

Cell junction,Cytoplasm,Focal adhesion

Background

The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES
GTX32286_20190131_ICCIF_19013101_937.jpg

GTX32286 ICC/IF Image

ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX32286 p130Cas (phospho Tyr249) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32286_20190131_WB_19013102_583.jpg

GTX32286 WB Image

WB analysis of EGF-treated HEK293T (A), EGF-treated NIH3T3 (B), EGF-treated Raw264.7 (C), EGF-treated PC12 (D) whole cell lysates using GTX32286 p130Cas (phospho Tyr249) antibody.

Package List Price ($)
$ 319