GeneTex
  • Country / Location Selection

United States (US)

p130Cas (phospho Tyr410) antibody

Anti-p130Cas (phospho Tyr410) antibody used in Western Blot (WB). GTX132162

Cat No. GTX132162

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

HeLa
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human p130Cas (phospho Tyr410). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BCAR1 scaffold protein, Cas family member,CAS,CAS1,CASS1,CRKAS,P130Cas

Cellular Localization

Cell junction , focal adhesion , Cytoplasm

Background

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-p130Cas (phospho Tyr410) antibody used in Western Blot (WB). GTX132162

GTX132162 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr410) antibody (GTX132162) diluted at 1:1000. (CIP: Calf-intestinal alkaline phosphatase)

REFERENCE
There are currently no references for p130Cas (phospho Tyr410) antibody (GTX132162). Be the first to share your publications with this product.
REVIEW

p130Cas (phospho Tyr410) antibody Cat No. GTX132162

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Sep-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : ARPE-19 cells
Blocking : 3% BSA and 0.02% Triton-X100 in PBS, 25°C, 1Hr
Primary Antibody : 1:50, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169