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p14ARF (isoform 3) antibody, Internal

Anti-p14ARF (isoform 3) antibody, Internal used in Western Blot (WB). GTX88429

Cat No. GTX88429

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88429-PEP).

Specificity/Sensitivity

This antibody is expected to recognize the pancreas isoform (NP_478104.1) only.

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ILRKKGRLPGEFSE, from the internal region of the protein sequence according to NP_478104.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

INK4 Antibody , P16INK4A Antibody , CMM2 Antibody , P19 Antibody , P14ARF Antibody , CDKN2A Antibody , P14 Antibody , MTS1 Antibody , CDK4I Antibody , P19ARF Antibody , TP16 Antibody , ARF Antibody , MLM Antibody , P16 Antibody , P16INK4 Antibody , INK4A Antibody , CDKN2 Antibody

Background

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]

Research Area

DATA IMAGES

Anti-p14ARF (isoform 3) antibody, Internal used in Western Blot (WB). GTX88429

GTX88429 WB Image

WB analysis of HeLa lysate using GTX88429 p14ARF (isoform 3) antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

There are currently no references for p14ARF (isoform 3) antibody, Internal (GTX88429). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for p14ARF (isoform 3) antibody, Internal (GTX88429). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf