p15 INK4b antibody

Cat. No. GTX55855

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all p15 INK4b products

Datasheet
Document

View product citations for antibody GTX55855 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
ICC/IF	1:100 - 1:500
IHC-P	1:100 - 1:200

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of p15 INK4b. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 2B , CDK4I , INK4B , MTS2 , P15 , TP15 , p15INK4b

Cellular Localization

Cytoplasm

Background

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1030 CDKN2B
UniProt: P42772 CDKN2B

Research Area

DATA IMAGES

GTX55855 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX55855 p15 INK4b antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX55855 WB Image

WB analysis of HepG2 (A), A549 (B), HeLa (C), mouse brain (D), rat kidney (E) whole cell lysates using GTX55855 p15 INK4b antibody.

GTX55855 ICC/IF Image

ICC/IF analysis of formalin-fixed A549 cells using GTX55855 p15 INK4b antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty