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p15 INK4b antibody

Anti-p15 INK4b antibody used in IHC (Paraffin sections) (IHC-P). GTX55855
Anti-p15 INK4b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55855
Anti-p15 INK4b antibody used in Western Blot (WB). GTX55855

Cat No. GTX55855

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of p15 INK4b. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase inhibitor 2B , CDK4I , INK4B , MTS2 , P15 , TP15 , p15INK4b

Cellular Localization

Cytoplasm

Background

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p15 INK4b antibody used in IHC (Paraffin sections) (IHC-P). GTX55855

GTX55855 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX55855 p15 INK4b antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-p15 INK4b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55855

GTX55855 ICC/IF Image

ICC/IF analysis of formalin-fixed A549 cells using GTX55855 p15 INK4b antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-p15 INK4b antibody used in Western Blot (WB). GTX55855

GTX55855 WB Image

WB analysis of HepG2 (A), A549 (B), HeLa (C), mouse brain (D), rat kidney (E) whole cell lysates using GTX55855 p15 INK4b antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319