GeneTex
United States (US)

p16 ARC antibody

GTX115342_40821_IHC_18121410_502.jpg
GTX115342_40821_WB_18121410_541.jpg

Cat No. GTX115342

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

THP-1 , HL-60

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p16 ARC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin Related Protein 2/3 Complex Subunit 5,Arc16,Dj127C7.3,P16-Arc,Arpc5

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection

Background

This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115342_40821_IHC_18121410_502.jpg

GTX115342 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ARPC5(GTX115342) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115342_40821_WB_18121410_541.jpg

GTX115342 WB Image

Sample (30 µg of whole cell lysate)
A: THP-1
B: HL-60
15% SDS PAGE
GTX115342 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169