APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , Neuro2A , Raw264.7 , C2C12
Predict Reactivity
Human, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.33 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human p18 INK4c. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886348
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cyclin dependent kinase inhibitor 2C , INK4C , p18 , p18-INK4C
Background
The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX130798 IHC-P Image
p18 INK4c antibody detects p18 INK4c protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.p18 INK4c stained by p18 INK4c antibody (GTX130798) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130798 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with p18 INK4c antibody (GTX130798) diluted at 1:500 and competitor's antibody (SC-865) diluted by 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX130798 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with p18 INK4c antibody (GTX130798) diluted at 1:500.
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GTX130798 IHC-P Image
p18 INK4c antibody detects p18 INK4c protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.p18 INK4c stained by p18 INK4c antibody (GTX130798) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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