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p21 Cip1 antibody [GT1032]

KO/KD Orthogonal
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Immunoprecipitation (IP). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Flow cytometry (FACS). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in IHC (Paraffin sections) (IHC-P). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

Cat. No. GTX629543

Reference 21
4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1032

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all p21 Cip1 products
PRODUCT

Summary

p21 (Cip1) antibody recognizes p21Cip1 protein, a cyclin-dependent kinase inhibitor with a predicted molecular weight of 18 kDa. p21 is a major downstream regulator of p53 activity in response to DNA damage to control cell cycle arrest. p21 also interacts with proliferating cell nuclear antigen (PCNA) to inhibit PCNA-dependent S-phase DNA synthesis.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
FACS Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HCT116 (30 μM Cisplatin treatment for 24 hr) , HCT116 (30 μM Cisplatin treatment for 48 hr)

Predict Reactivity

Sheep, Bovine, Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888121

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1A , CAP20 , CDKN1 , CIP1 , MDA-6 , P21 , SDI1 , WAF1 , p21CIP1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

KO/KD

Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

Orthogonal

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein by western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract (untreated)
B. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 24 hr)
C. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 48 hr)
15% SDS-PAGE
p21 Cip1 antibody [GT1032] (GTX629543) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p21 Cip1 antibody [GT1032] used in Immunoprecipitation (IP). GTX629543

GTX629543 IP Image

p21 Cip1 antibody [GT1032] immunoprecipitates CDKN1A protein in IP experiments.
IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr
A. 30 μg HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr
B. Control with 4 μg of preimmune Mouse IgG
C. Immunoprecipitation of CDKN1A protein by 4 μg p21 Cip1 antibody [GT1032] (GTX629543)
15 % SDS-PAGE
The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543

GTX629543 ICC/IF Image

Orthogonal

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543

GTX629543 ICC/IF Image

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK4 protein stained by CDK4 antibody (GTX102993) diluted at 1:1000.
Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-p21 Cip1 antibody [GT1032] used in Flow cytometry (FACS). GTX629543

GTX629543 FACS Image

p21 Cip1 antibody [GT1032] (GTX629543) detects p21 Cip1 by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: p21 Cip1 antibody [GT1032] (GTX629543) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-p21 Cip1 antibody [GT1032] used in IHC (Paraffin sections) (IHC-P). GTX629543

GTX629543 IHC-P Image

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
p21 Cip1 stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25961745

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25961745

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25961745

REFERENCE

Application Reference

Hsueh YH et al. J Orthop Translat 2023; 38 : 141-155 Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Ming-Lin Tsai et al. FEBS Open Bio 2022; 12 (5) : 993-1005 CRISPR-mediated knockout of VEGFR2/KDR inhibits cell growth in a squamous thyroid cancer cell line.
Application : WB
Reactivity : Human
Zanr? V et al. Int J Mol Sci. 2022; 23 (3) : 1121 Salviolone from Salvia miltiorrhiza Roots Impairs Cell Cycle Progression, Colony Formation, and Metalloproteinase-2 Activity in A375 Melanoma Cells: Involvement of P21(Cip1/Waf1) Expression and STAT3 Phosphorylation.
Application : WB
Reactivity : Human
Ya-Hui Chen et al. Cancers (Basel) 2021; 13 (18)
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

p21 Cip1 antibody [GT1032] Cat. No. GTX629543

Rating
( Average 4.5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.3 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Date : Anonymous submitted on 14-Oct-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 20 μg
Blocking : 5% non-fat milk in TBST, 22°C, 0.5Hr
Primary Antibody : 1:2000, 22°C, 18Hr
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Date : Anonymous submitted on 13-Mar-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : KOPN79 cells
Amount used : 20 μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543
Date : Anonymous submitted on 03-Jan-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : MIA PaCa-2 cells
Blocking : 3% BSA, 25°C, 0.3Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 0.8% Triton X-100 in 3% BSA
Primary Antibody : 1:100, 4°C, 16Hr
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Date : Anonymous submitted on 27-Aug-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : HCT116 cells
Amount used : 30μg
Blocking : 1%BSA in PBST, 4°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Submit a Review
Application Reference
Hsueh YH et al. J Orthop Translat 2023; 38:141-155 Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Ming-Lin Tsai et al. FEBS Open Bio 2022; 12 (5):993-1005 CRISPR-mediated knockout of VEGFR2/KDR inhibits cell growth in a squamous thyroid cancer cell line.
Application : WB
Reactivity : Human
Zanr? V et al. Int J Mol Sci. 2022; 23 (3):1121 Salviolone from Salvia miltiorrhiza Roots Impairs Cell Cycle Progression, Colony Formation, and Metalloproteinase-2 Activity in A375 Melanoma Cells: Involvement of P21(Cip1/Waf1) Expression and STAT3 Phosphorylation.
Application : WB
Reactivity : Human
Ching-Ling Lin et al. Biomol Ther (Seoul) 2021; 29 (5):551-561 Platelet-Derived Growth Factor Receptor-α Subunit Targeting Suppresses Metastasis in Advanced Thyroid Cancer In Vitro and In Vivo.
Application : WB
Reactivity : Human
Ya-Hui Chen et al. Cancers (Basel) 2021; 13 (18)
Application : WB
Reactivity : Human
Pons BJ et al. Cell Mol Life Sci 2021; 78 (17-18):6319-6335 Chronic exposure to Cytolethal Distending Toxin (CDT) promotes a cGAS-dependent type I interferon response.
Application : WB
Reactivity : Human
Li R et al. Invest Ophthalmol Vis Sci 2021; 62 (9):2 Molecular Hydrogen Attenuated N-methyl-N-Nitrosourea Induced Corneal Endothelial Injury by Upregulating Anti-Apoptotic Pathway.
Application : ICC/IF
Reactivity : Human
Chen YH et al. Cancer Manag Res 2020; 12:4645-4665
Application : WB
Reactivity : Human
Hsu WH et al. Aging (Albany NY) 2020; 12 (11):10556-10577 T1-11, an adenosine derivative, ameliorates aging-related behavioral physiology and senescence markers in aging mice.
Application : WB
Reactivity : Human
Yu S et al. Int Immunopharmacol 2020; 81:106294 M1 macrophages accelerate renal glomerular endothelial cell senescence through reactive oxygen species accumulation in streptozotocin-induced diabetic mice.
Application : WB
Chen CY et al. Front Oncol 2020; 10:216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.
Application : WB
Reactivity : Human
Chueh-Hsuan Lu et al. bioRxiv 2019; (Epub) Application of non-invasive low-intensity pulsed electric field with thermal cycling-hyperthermia for synergistically enhanced anticancer effect of chlorogenic acid on PANC-1 cells.
Application : WB
Reactivity : Human
Chen KY et al. PLoS One 2019; 14 (7):e0219317 Resveratrol induced premature senescence and inhibited epithelial-mesenchymal transition of cancer cells via induction of tumor suppressor Rad9.
Application : WB
Reactivity : Human
Li HY et al. J Agric Food Chem 2019; 67 (7):1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Application : WB
Reactivity : Mouse
Hsu KW et al. Int J Mol Sci 2018; 19 (2) The Application of Non-Invasive Apoptosis Detection Sensor (NIADS) on Histone Deacetylation Inhibitor (HDACi)-Induced Breast Cancer Cell Death.
Huang LC et al. Dis Markers 2018; 2018:3835783 CRISPR/Cas9 Genome Editing of Epidermal Growth Factor Receptor Sufficiently Abolished Oncogenicity in Anaplastic Thyroid Cancer.
Tian YF et al. Am J Cancer Res 2018; 8 (9):1812-1822 Preprogramming therapeutic response of PI3K/mTOR dual inhibitor via the regulation of EHMT2 and p27 in pancreatic cancer.
Application : WB
Reactivity : Human
Huang L.C. et al. Disease Markers 2018; 2018:Article ID 3835783 CRISPR/Cas9 Genome Editing of Epidermal Growth Factor Receptor Sufficiently Abolished Oncogenicity in Anaplastic Thyroid Cancer.
Application : WB
Reactivity : Human
Chang TC et al. PLoS One 2015; 10 (5):e0126537 High glucose induces bone marrow-derived mesenchymal stem cell senescence by upregulating autophagy.
Application : WB
Reactivity : Human
Chung CL et al. Evid Based Complement Alternat Med 2015; 2015:595824 Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes.
Application : WB
Reactivity : Mouse
SDS
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Custom Conjugations