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p21 Cip1 antibody [GT1032]

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Immunoprecipitation (IP). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543

Cat No. GTX629543

Host Mouse
Clonality Monoclonal
Clone Name GT1032
Isotype IgG2a
Application WB, ICC/IF, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HeLa , HCT116 (30 μM Cisplatin treatment for 24 hr) , HCT116 (30 μM Cisplatin treatment for 48 hr)

Predict Reactivity

Sheep, Bovine, Cat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cyclin dependent kinase inhibitor 1A,CAP20,CDKN1,CIP1,MDA-6,P21,SDI1,WAF1,p21CIP1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein by western blot analysis.
A. 30 µg HCT116 whole cell lysate/extract (untreated)
B. 30 µg HCT116 whole cell lysate/extract (30 µM Cisplatin treatment for 24 hr)
C. 30 µg HCT116 whole cell lysate/extract (30 µM Cisplatin treatment for 48 hr)
15% SDS-PAGE
p21 Cip1 antibody [GT1032] (GTX629543) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p21 Cip1 antibody [GT1032] used in Immunoprecipitation (IP). GTX629543

GTX629543 IP Image

p21 Cip1 antibody [GT1032] immunoprecipitates CDKN1A protein in IP experiments.
IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr
A. 30 μg HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr
B. Control with 4 μg of preimmune Mouse IgG
C. Immunoprecipitation of CDKN1A protein by 4 μg p21 Cip1 antibody [GT1032] (GTX629543)
15 % SDS-PAGE
The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]

Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543

GTX629543 ICC/IF Image

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK4 protein stained by CDK4 antibody (GTX102993) diluted at 1:1000.
Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543

GTX629543 ICC/IF Image

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE
REVIEW

p21 Cip1 antibody [GT1032] Cat No. GTX629543

Rating ( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Mar-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : KOPN79 cells
Amount used : 20 μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 03-Jan-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : MIA PaCa-2 cells
Blocking : 3% BSA, 25°C, 0.3Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 0.8% Triton X-100 in 3% BSA
Primary Antibody : 1:100, 4°C, 16Hr
Date : Anonymous submitted on 27-Aug-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : HCT116 cells
Amount used : 30μg
Blocking : 1%BSA in PBST, 4°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169